Author Affiliations
Abstract
1 State Key Laboratory of Precision Spectroscopy, Department of Physics, and Department of Chemistry, East China Normal University, Shanghai 200062
2 Department of Physics and Electronics, Henan University, Kaifeng 475003
Dependence of surface-enhanced Raman scattering (SERS) from Calf thymus DNA on anions is investigated. With the silver colloid, the bands at 732, 960 and 1333 cm-1 for adenine (A), 1466 cm-1 for deoxyribose, and 1652 cm-1 for the C=O group of thymine (T) are observably enhanced. With the presence of the Cl- or SO^(2-)_(4) anions, the bands at 732 and 1326/1329 cm-1 for the symmetric stretching and skeletal vibrational modes of adenine (A) are dramatically enhanced, and the enhancement effect with the SO^(2-)_(4) ion is more than that with the Cl_ ion. The experimental results show that the DNA molecule can be adsorbed on the silver colloid particles through the C6N and N7 of adenine (A), the C=O of thymine (T) and deoxyribose. Moreover, the formed hydrogen bonding of the Cl_ or SO^(2-)_(4) ions to the C6NH2 group of adenine (A) can induce larger C6N electronegativity, which is favor for the C6N/N7 cooperative adsorption on the (Ag)^(+)_(n) colloid particles.
表面增强拉曼散射 阴离子影响 小牛胸腺DNA 氯离子 硫酸根离子 170.5660 Raman spectroscopy 300.6450 Spectroscopy, Raman 290.5860 Scattering, Raman 170.0170 Medical optics and biotechnology Chinese Optics Letters
2008, 6(7): 526
